ELISpot Flex: Bovine IL-2 (ALP)

3111-2A

Publications: 0

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Our most adaptable ELISpot format, made to fit into your unique plan. Combine it with a substrate, plate, and/or antigen of choice.

Components

Capture mAb	MT11A31
Detection mAb	MT3B3, biotin
Enzyme conjugate	Streptavidin-ALP

In stock

Delivery 4-9 business days

Shipping $0

Complementary products

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ELISpot substrate: BCIP/NBT-plus for ALP

Product specifications

Intended use

This ELISpot Flex kit is intended for the enumeration of cells secreting bovine IL-2 using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

Product	ELISpot Flex: Bovine IL-2 (ALP)
Application	ELISpot
Analyte	IL-2
Reactivity	Cow
Specificity	This kit is based on a matched pair of mAbs specific for bovine (cow) IL-2.

Shipping and Storage

Shipping	Shipped at ambient temperature.
Storage	Store at 4-8 °C upon receipt.
Shelf life	At least 18 months from date of receipt.

Performance

PBMC from cow were incubated in the presence or absence of Phytohaemagglutinin (PHA) and the number of cells secreting IL-2 was analyzed by bovine IL-2 ELISpot.

Documents

Datasheet/Protocol
SDS

Tutorials

Tutorial, Published December 28, 2023

Incubation strategies for success in ELISpot and FluoroSpot

We've made a handy table summarizing incubation times for different analytes to make your research planning for the ELISpot and FluoroSpot assay a little easier.

Tutorial, Published November 24, 2022

Cell stimulation in ELISpot and FluoroSpot

In this feature, we’ll walk you through the optimal use of different polyclonal and antigen-specific stimuli.

Tutorial, Published November 7, 2022

7 common ELISpot errors and how to avoid them

ELISpot troubleshooting. We discuss 7 of the most common issues we’ve encountered in ELISpot, what mistakes causes them and how to avoid and correct them.

Tutorial, Published October 14, 2022

What is a positive ELISpot response?

DFR is a non-parametric statistical test for determining positive responses in ELISpot and FluoroSpot.

Publications (0)

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Analyte information

IL-2

Analyte description	Interleukin 2 (IL-2) is predominantly produced by activated T cells. IL-2 promotes the proliferation, differentiation, and survival of antigen-activated T cells and NK cells. After antigen stimulation, the cytokine also promotes the differentiation of T cells into effector and memory T cells. Therefore IL-2 plays an important role in the body's response to infection. IL-2 is also key to tolerance, as it promotes the differentiation of certain immature T cells into regulatory T cells in the thymus, ultimately preventing autoimmune diseases.
Alternative names	Interleukin-2, Interleukin 2, IL-2, IL2, T cell growth factor, TCGF
Cell type	T cell, Th1, Th2, Th17, Tfh
Gene ID	280822